Browsing by Author "Abazović D."

Now showing 1 - 3 of 3

Neutrophil-to-Lymphocyte Ratio, Monocyte-to-Lymphocyte Ratio, Platelet-to-Lymphocyte Ratio, and Mean Platelet Volume-to-Platelet Count Ratio as Biomarkers in Critically Ill and Injured Patients: Which Ratio to Choose to Predict Outcome and Nature of Bacteremia?
(2018) Djordjevic, Dragan; Rondović G.; Surbatović M.; Stanojevic, Ivan; Udovicic I.; Andjelic T.; Zeba S.; Milosavljevic D.; Stankovic, Nevena; Abazović D.; Jevdjic, Jasna; Vojvodic, Danilo
© 2018 Dragan Djordjevic et al. Background. Neutrophil-to-lymphocyte ratio (NLR), monocyte-to-lymphocyte ratio (MLR), platelet-to-lymphocyte ratio (PLR), and mean platelet volume-to-platelet count (MPV/PC) ratio are readily available parameters that might have discriminative power regarding outcome. The aim of our study was to assess prognostic value of these biomarkers regarding outcome in critically ill patients with secondary sepsis and/or trauma. Methods. A total of 392 critically ill and injured patients, admitted to surgical ICU, were enrolled in a prospective observational study. Leukocyte and platelet counts were recorded upon fulfilling Sepsis-3 criteria and for traumatized Injury Severity Score > 25 points. Patients were divided into four subgroups: peritonitis, pancreatitis, trauma with sepsis, and trauma without sepsis. Results. NLR and MPV/PC levels were significantly higher in nonsurvivors (AUC/ROC of 0.681 and 0.592, resp., in the peritonitis subgroup; 0.717 and 0.753, resp., in the pancreatitis subgroup); MLR and PLR did not differ significantly. There was no significant difference of investigated biomarkers between survivors and nonsurvivors in trauma patients with and without sepsis except for PLR in the trauma without sepsis subgroup (significantly higher in nonsurvivors, AUC/ROC of 0.719). Independent predictor of lethal outcome was NLR in the whole cohort and in the peritonitis subgroup as well as MPV in the pancreatitis subgroup. Also, there were statistically significant differences in MPV/PC, MLR, and PLR values regarding nature of bacteremia. In general, the lowest levels had been found in patients with Gram-positive blood cultures. Conclusions. NLR and MPV were very good independent predictors of lethal outcome. For the first time, we demonstrate that nature of bacteremia influences MPV/PC, MLR, and PLR. In heterogeneous cohort subgroup, analysis is essential.
The effect of dental caries and restorative biomaterials on IL-1 β and TNF-α levels in the gingival crevicular fluid
(2021) Stefanovic V.; Taso E.; Kanjevac, Tatjana; Abazović D.; Rakić M.; Petković Ćurčin A.; Acovic A.; Vojvodic, Danilo
© 2021 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved. Background/Aim. In the spirit of personalized medicine, de-termining caries biomarkers in the saliva and gingival crevicular fluid (GCF) attracts great attention in the current dental re-search. The concentration of GCF cytokines is illustrative in depicting the processes in tooth structures. Their relevance must be inspected with aspects of tooth position and caries le-sion level. Different impacts of dental restoration materials on GCF IL-1β and TNF-α could be used as a parameter for esti-mating local inflammation. This paper aimed to estimate the concentrations of the proinflammatory cytokines (IL-1β and TNF-α) in the GCF and to correlate them with caries exten-sion, tooth position, and different restorative biomaterials. Methods. GCF samples were collected from 90 periodontally healthy patients demonstrating at least one tooth with proximal caries and one intact tooth, at the baseline, 7 and 30-days post-treatment. The biomarkers' profile was investigated in relation to different levels of caries extension (superficial, pulpitis, gan-grenous, root affection), defect size, and restorative biomaterial. Results. Before therapy, caries level was significantly associat-ed with GCF IL-1β concentration, demonstrating the lowest level in gangrenous (C4) and superficial caries (C2). Thirty days after therapy, root affection (C5) was characterized by the high-est IL-1β concentration. Different dental fillings showed vari-ous GCF cytokine changes. CPC induced a significant IL-1β increase in more than 70% of treated patients. Caries lesion size was insignificantly associated with GCF levels of these proin-flammatory cytokines, where larger defects were followed by an average cytokine increase. Considering the tooth position be-fore therapy, IL-1β had the highest level in GCF samples from caries-affected canines and second molars, while TNF-α showed the highest levels from canines GCF. Dental restora-tion induced cytokine increase in canines (IL-1β and TNF-α), 1st and 2nd molars GCF (IL-1β). Conclusion. Inflammation intensity of tooth structures was directly reflected in IL-1β and TNF-α concentrations. Dental restoration significantly affects IL-1β and TNF-α levels, depending on the used dental filling-type material. The profile of these cytokines varied in GCF samples of the tooth with different anatomical positions, where canines and molars demonstrated the highest level. An increase of these proinflammatory cytokines in the absence of any symptomatic manifestation of the inflammatory response can be considered as a possible tooth reparation parameter.
Vitamin D receptor gene variants contribute to hip and knee osteoarthritis susceptibility
(2021) Vranic V.; Zeljic K.; Stefik D.; Ivković, Nikola; Abazović D.; Arsenijevic, Nebojsa; Vojvodic, Danilo; Supic, Gordana
Vitamin D receptor (VDR) gene polymorphisms could play a significant role in the susceptibility and patho-genesis of osteoarthritis (OA), the most common degenerative joint disorder in humans. The current study involved 94 OA patients and 100 healthy, asymptomatic controls. VDR variants FokI (rs2228570), TaqI (rs731236), ApaI (rs7975232) and EcoRV (rs4516035) were genotyped using TaqMan-based real-time PCR. Adjusted odds ratio (OR) analysis showed that VDR TaqI and FokI polymorphisms are significantly associated with susceptibility to OA (OR=1.986, P=0.001 and OR=1.561, P=0.017, respectively). Joint-specific analysis showed that the VDR TaqI polymorphism was associated with risk of hip OA (OR=1.930, P=0.005) and knee OA (OR=1.916, P=0.028), while the VDR FokI polymorphism was associated with higher risk of knee OA (OR=2.117, P=0.012). VDR TaqI and FokI polymorphisms are associated with the occurrence of persistent pain (P=0.005 and P=0.027, respectively), while ApaI was associated with a family history of OA (p=0.004). The VDR FokI and TaqI genetic variants significantly contribute to osteoarthritis susceptibility, the occurrence of persistent pain, and potentially to joint-specific OA risk.